Key features and details
- Rat monoclonal [MAC 237] to Actin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Waterbug
- Isotype: IgG2b
Product nameAnti-Actin antibody [MAC 237]
See all Actin primary antibodies
DescriptionRat monoclonal [MAC 237] to Actin
SpecificityAntibody reacts with actin in Lethocerus and Drosophila flight and non-flight muscle and with actin in flight muscles of all other insect species tested. Also reacts with mammalian actin (tested vs. rat). Antibody reacts with arthrin in flight muscles of Hemiptera and Diptera.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Drosophila melanogaster, Waterbug
Flight muscle extract from Lethocerus indicus (Waterbug)
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Clone numberMAC 237
Our Abpromise guarantee covers the use of ab50591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- a-actin antibody
- ACTA antibody
ab50591 staining Actin in mouse proximal colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% NDS for 24 hours at 4°C; antigen retrieval was by heat mediation in buffer, pH9. Samples were incubated with primary antibody (1/100 in 5% BSA/NDS) for 24 hours at 4°C. An undiluted Alexa Fluor® 647-conjugated donkey anti-rat IgG polyclonal was used as the secondary antibody.
Also stained with ab76055 (green) at 1/100.
IHC image of Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50591, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab50591 has been referenced in 8 publications.
- Song X et al. Zi Shen Huo Luo Formula Enhances the Therapeutic Effects of Angiotensin-Converting Enzyme Inhibitors on Hypertensive Left Ventricular Hypertrophy by Interfering With Aldosterone Breakthrough and Affecting Caveolin-1/Mineralocorticoid Receptor Colocalization and Downstream Extracellular Signal-Regulated Kinase Signaling. Front Pharmacol 11:383 (2020). PubMed: 32317965
- Haihua C et al. Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage. Sci Rep 8:10346 (2018). PubMed: 29985461
- Wu X et al. Morphological and functional changes of microglia cultured under different oxygen concentrations and the analysis of related mechanisms. Exp Ther Med 15:2015-2019 (2018). PubMed: 29434798
- Holvoet B et al. Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice. Stem Cell Reports 5:1183-1195 (2015). IHC . PubMed: 26626179
- Shaw WR et al. Mating activates the heme peroxidase HPX15 in the sperm storage organ to ensure fertility in Anopheles gambiae. Proc Natl Acad Sci U S A 111:5854-9 (2014). WB . PubMed: 24711401
- Idrissi FZ et al. Ultrastructural dynamics of proteins involved in endocytic budding. Proc Natl Acad Sci U S A 109:E2587-94 (2012). Electron Microscopy ; Saccharomyces cerevisiae . PubMed: 22949647
- Wolff G et al. Neuronal organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus: correspondence with the mushroom body ground pattern. J Comp Neurol 520:2824-46 (2012). PubMed: 22547177
- Lakey A et al. Identification and localization of high molecular weight proteins in insect flight and leg muscle. EMBO J 9:3459-67 (1990). PubMed: 2209553