Overview

  • Product name

    Actin Polymerization/Depolymerization Assay Kit
  • Detection method

    Fluorescent
  • Product overview

    Actin Polymerization/Depolymerization Assay Kit (ab239724) can be used to study the effect of different compounds, proteins and tissue extracts on Actin polymerization and depolymerization.


    The kit utilizes a proprietary Pyrene-labeled Actin molecule that develops a higher fluorescent signal if it undergoes polymerization. The signal can be easily detected using a fluorescence microplate reader. The assay is simple, high- throughput compatible, and can be completed in less than three hours.


    Samples: Protein, Tissue Extracts, Compounds/Chemotherapeutic Agents. 

  • Platform

    Microplate reader

Properties

Images

  • The process is inhibited by Latrunculin A (23 μM). Assays were performed following the kit protocol. Note: Latrunculin A is Actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin. Latrunculin A acts a depolymerization agent acting on Actin filaments (Factin).

  • Assays were performed following the kit protocol. Note: Latrunculin A is Actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin. Latrunculin A acts a depolymerization agent acting on Actin filaments (Factin).

Protocols

References

ab239724 has not yet been referenced specifically in any publications.

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