Overview

  • Product name

    Anti-Actin Regulatory Protein CAPG/MCP antibody
    See all Actin Regulatory Protein CAPG/MCP primary antibodies
  • Description

    Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human Actin Regulatory Protein CAPG/MCP aa 157-333.
    Database link: P40121

  • Positive control

    • A549, HeLa, HepG2, HCT116 and NIH 3T3 whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
  • General notes

     This product was previously labelled as Actin Regulatory Protein CAPG

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity

    Macrophages and macrophage-like cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin capping protein GCAP39 antibody
    • Actin regulatory protein CAP G antibody
    • Actin regulatory protein CAP-G antibody
    • AFCP antibody
    • Capg antibody
    • CAPG_HUMAN antibody
    • Capping actin protein gelsolin like antibody
    • Capping protein (actin filament) gelsolin like antibody
    • Capping protein gelsolin like antibody
    • Epididymis secretory protein Li 66 antibody
    • gCap39 antibody
    • Gelsolin like capping protein antibody
    • HEL S 66 antibody
    • Macrophage capping protein antibody
    • Macrophage-capping protein antibody
    • mbh1 antibody
    • MCP antibody
    • Myc basic motif homolog 1 antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Actin Regulatory Protein CAPG/MCP with ab155688 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) at 1/2000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling Actin Regulatory Protein CAPG/MCP with ab155688 at 1/500 dilution.

References

This product has been referenced in:

  • Kumar A  et al. Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation. Mol Cell Endocrinol 404:91-101 (2015). ICC/IF ; Rat . Read more (PubMed: 25637714) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Testis sections)
Permeabilization
Yes - 0.1% Tween20
Specification
Testis sections
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 16 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (testis)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Anita Kumar

Verified customer

Submitted Feb 25 2014

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