Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688)
Key features and details
- Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Actin Regulatory Protein CAPG/MCP antibody
See all Actin Regulatory Protein CAPG/MCP primary antibodies -
Description
Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human Actin Regulatory Protein CAPG/MCP aa 157-333.
Database link: P40121 -
Positive control
- A549, HeLa, HepG2, HCT116 and NIH 3T3 whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
Tissue specificity
Macrophages and macrophage-like cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 353121 Cow
- Entrez Gene: 822 Human
- Entrez Gene: 12332 Mouse
- Entrez Gene: 297339 Rat
- Omim: 153615 Human
- SwissProt: Q865V6 Cow
- SwissProt: P40121 Human
- SwissProt: P24452 Mouse
see all -
Alternative names
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
Images
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Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Actin Regulatory Protein CAPG/MCP with ab155688 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Anti-Actin Regulatory Protein CAPG/MCP antibody (ab155688) at 1/2000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling Actin Regulatory Protein CAPG/MCP with ab155688 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab155688 has been referenced in 4 publications.
- Yi L et al. Downregulation of NSD3 (WHSC1L1) inhibits cell proliferation and migration via ERK1/2 deactivation and decreasing CAPG expression in colorectal cancer cells. Onco Targets Ther 12:3933-3943 (2019). PubMed: 31190890
- Zhang X et al. Ferroptosis is governed by differential regulation of transcription in liver cancer. Redox Biol 24:101211 (2019). PubMed: 31108460
- Fu Q et al. Analysis of clinical characteristics of macrophage capping protein (CAPG) gene expressed in glioma based on TCGA data and clinical experiments. Oncol Lett 18:1344-1350 (2019). PubMed: 31423196
- Kumar A et al. Estrogen and androgen regulate actin-remodeling and endocytosis-related genes during rat spermiation. Mol Cell Endocrinol 404:91-101 (2015). ICC/IF ; Rat . PubMed: 25637714