Overview

  • Product name

    Anti-Actin Regulatory Protein CAPG/MCP antibody
    See all Actin Regulatory Protein CAPG/MCP primary antibodies
  • Description

    Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Actin Regulatory Protein CAPG/MCP aa 298-348. The exact sequence is proprietary.
    Sequence:

    GRKANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGHESPIF KQFFKDWK


    Database link: P40121

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysates.
  • General notes

     This product was previously labelled as Actin Regulatory Protein CAPG

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab217064 was affinity purified using an epitope specific to CAPG immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity

    Macrophages and macrophage-like cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin capping protein GCAP39 antibody
    • Actin regulatory protein CAP G antibody
    • Actin regulatory protein CAP-G antibody
    • AFCP antibody
    • Capg antibody
    • CAPG_HUMAN antibody
    • Capping actin protein gelsolin like antibody
    • Capping protein (actin filament) gelsolin like antibody
    • Capping protein gelsolin like antibody
    • Epididymis secretory protein Li 66 antibody
    • gCap39 antibody
    • Gelsolin like capping protein antibody
    • HEL S 66 antibody
    • Macrophage capping protein antibody
    • Macrophage-capping protein antibody
    • mbh1 antibody
    • MCP antibody
    • Myc basic motif homolog 1 antibody
    see all

Images

  • Detection of Actin Regulatory Protein CAPG/MCP in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217064 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217064 was used at 0.4 µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

  • All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab217064) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 30 seconds

References

ab217064 has not yet been referenced specifically in any publications.

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