Overview

  • Product name

    Anti-Actin Regulatory Protein CAPG/MCP antibody
    See all Actin Regulatory Protein CAPG/MCP primary antibodies
  • Description

    Goat polyclonal to Actin Regulatory Protein CAPG/MCP
  • Host species

    Goat
  • Specificity

    ab223631 is expected to recognize both reported isoforms (NP_001738.2; NP_001243069.1). Reported variants represent identical protein: NP_001243068.1, NP_001738.2.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Actin Regulatory Protein CAPG/MCP aa 147-159 (internal sequence) (Cysteine residue). (NP_001738.2).
    Sequence:

    C-NIRATERALNWDS


    Database link: P40121

  • Positive control

    • WB: Human spleen and peripheral blood lymphocyte lysates; U937 cell lysate.
  • General notes

     This product was previously labelled as Actin Regulatory Protein CAPG

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223631 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
  • Tissue specificity

    Macrophages and macrophage-like cells.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 3 gelsolin-like repeats.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin capping protein GCAP39 antibody
    • Actin regulatory protein CAP G antibody
    • Actin regulatory protein CAP-G antibody
    • AFCP antibody
    • Capg antibody
    • CAPG_HUMAN antibody
    • Capping actin protein gelsolin like antibody
    • Capping protein (actin filament) gelsolin like antibody
    • Capping protein gelsolin like antibody
    • Epididymis secretory protein Li 66 antibody
    • gCap39 antibody
    • Gelsolin like capping protein antibody
    • HEL S 66 antibody
    • Macrophage capping protein antibody
    • Macrophage-capping protein antibody
    • mbh1 antibody
    • MCP antibody
    • Myc basic motif homolog 1 antibody
    see all

Images

  • All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab223631) at 0.3 µg/ml

    Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate (in RIPA buffer)
    Lane 2 : Human spleen lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Primary incubation was 1 hour.

  • Anti-Actin Regulatory Protein CAPG/MCP antibody (ab223631) at 0.3 µg/ml + human peripheral blood lymphocytes (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223631 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223631.
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