Key features and details
- Rabbit polyclonal to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Actin Regulatory Protein CAPG/MCP antibody
See all Actin Regulatory Protein CAPG/MCP primary antibodies
DescriptionRabbit polyclonal to Actin Regulatory Protein CAPG/MCP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide corresponding to Human Actin Regulatory Protein CAPG/MCP aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Actin Regulatory Protein CAPG/MCP (ab95385) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HL60; U937; THP1; RAW 264.7.
This product was previously labelled as Actin Regulatory Protein CAPG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab90873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
FunctionCalcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Tissue specificityMacrophages and macrophage-like cells.
Sequence similaritiesBelongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody (ab90873) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab90873 has not yet been referenced specifically in any publications.