Recombinant Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13194-34] to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34]
See all Actin Regulatory Protein CAPG/MCP primary antibodies -
Description
Rabbit monoclonal [EPR13194-34] to Actin Regulatory Protein CAPG/MCP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: THP-1, U937 and HeLa cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13194-34 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
|
Notes |
---|
WB
1/1000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa). |
Target
-
Function
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
Tissue specificity
Macrophages and macrophage-like cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 822 Human
- Entrez Gene: 12332 Mouse
- Entrez Gene: 297339 Rat
- Omim: 153615 Human
- SwissProt: P40121 Human
- SwissProt: P24452 Mouse
- SwissProt: Q6AYC4 Rat
- Unigene: 516155 Human
see all -
Alternative names
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
Images
-
All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAPG knockout HeLa cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : U-937 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab181095 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181095 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used. Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE. ab181095 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/50000 dilution + U937 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab181095 has not yet been referenced specifically in any publications.