Product nameAnti-activated Notch1 antibody
See all activated Notch1 primary antibodies
DescriptionRabbit polyclonal to activated Notch1
Specificityab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.
Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
- Extracts from NIH 3T3 cells, treated with etoposide (25µM, 1hour). IHC-P: Human brain tissue
ab52301 is cleaved at Val 1744
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
PBS without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab52301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24098462|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20680961|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).|
FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A.
Tissue specificityIn fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Involvement in diseaseDefects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
Sequence similaritiesBelongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.
Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD.
Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
- Information by UniProt
- hN1 antibody
- Neurogenic locus Notch homolog protein 1 antibody
- NICD antibody
Immunofluorescence analysis of frozen tissue sections (10µm) of intestine from sham operated rats (control group) or rats after Ischemic-Reperfusion (I/R) injury labeling Notch1 with ab52301. Tissue sections were frozen after being fixed with 4% formaldehyde, then incubated in 3% H2O2 for 30 minutes and then blocked with 5% BSA for 30 mins at room temperature. The sections were incubated overnight with ab52301 (PBS + 3% BSA). An appropriate secondary antibody was then added. DAPI (in blue) was used to stain the nucleus.
All lanes : Anti-activated Notch1 antibody (ab52301) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour)
Lane 2 : NIH 3T3 cell extract treated with etoposide
(25µM, 1 hour) with immunising peptide
Predicted band size: 272 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.
This product has been referenced in:
- Liu W et al. Mesenchymal stem cells alleviate the early brain injury of subarachnoid hemorrhage partly by suppression of Notch1-dependent neuroinflammation: involvement of Botch. J Neuroinflammation 16:8 (2019). Read more (PubMed: 30646897) »
- Zhou H et al. Paeonol reverses promoting effect of the HOTAIR/miR-124/Notch1 axis on renal interstitial fibrosis in a rat model. J Cell Physiol 234:14351-14363 (2019). Read more (PubMed: 30714138) »