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ab52301 is cleaved at Val 1744
Our Abpromise guarantee covers the use of ab52301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24098462|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20680961|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).|
Immunofluorescence analysis of frozen tissue sections (10µm) of intestine from sham operated rats (control group) or rats after Ischemic-Reperfusion (I/R) injury labeling Notch1 with ab52301. Tissue sections were frozen after being fixed with 4% formaldehyde, then incubated in 3% H2O2 for 30 minutes and then blocked with 5% BSA for 30 mins at room temperature. The sections were incubated overnight with ab52301 (PBS + 3% BSA). An appropriate secondary antibody was then added. DAPI (in blue) was used to stain the nucleus.
Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling activated Notch1 with ab52301 at a dilution of 1/100. The picture on the right is treated with the synthesized peptide.
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