Anti-Caspase-3 antibody (ab49822)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Caspase-3 antibody
See all Caspase-3 primary antibodies -
Description
Rabbit polyclonal to Caspase-3 -
Host species
Rabbit -
Specificity
The immunogen for this product is aa167-175 within the p17 subunit (cleaved caspase) but because this is a poly the epitope will vary and not all batches will be flush against the cleavage site.
This means it will detect a number of additional cleavage products in addition to p17:
• P12+17,
• P12+P17+propeptide 1
• p12+p17+proppetide 1 + propeptide 2,
• P17+propeptide 1,
• p17+propeptide 1 + propeptide 2 -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Caspase-3 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: P42574 -
Positive control
- ICC/IF: Human epitheloid carcinoma HeLa cell line, treated with staurosporine and WB: Human recombinant caspase-3, active.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion exchange chromatography. The resulting IgG fraction is further purified by absorption on the Caspase-3 peptide (Human, amino acids 167-178) corresponding to the uncleaved Caspase 3 site sequence. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500.
|
ICC/IF |
1/1000.
|
Notes |
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WB
1/500. |
ICC/IF
1/1000. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
Tissue specificity
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
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Alternative names
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
Images
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Anti-Caspase-3 antibody (ab49822) at 1/1000 dilution
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
Developed using the ECL technique.
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cleaved Caspase-3 with ab49822 at 1/1000. HeLa cells were treated with 1 μg/mL staurosporine, fixed and permeabilized with Methanol followed by Acetone. Goat Anti-Rabbit IgG (whole molecule)-FITC was used as the secondary antibody. Cells were counterstained with DAPI (blue) to stain nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (388)
ab49822 has been referenced in 388 publications.
- Song BW et al. Cold-pressed oil from Citrus aurantifolia inhibits the proliferation of vascular smooth muscle cells via regulation of PI3K/MAPK signaling pathways. Exp Ther Med 23:21 (2022). PubMed: 34815773
- Dai HT et al. microRNA-375 inhibits the malignant behaviors of hepatic carcinoma cells by targeting NCAPG2. Neoplasma 69:16-27 (2022). PubMed: 34818025
- Jiang J et al. Hyperoxia induces alveolar epithelial cell apoptosis by regulating mitochondrial function through small mothers against decapentaplegic 3 (SMAD3) and extracellular signal-regulated kinase 1/2 (ERK1/2). Bioengineered 13:242-252 (2022). PubMed: 34898379
- Wu Q et al. A Single Administration of Riluzole Applied Acutely After Spinal Cord Injury Attenuates Pro-inflammatory Activity and Improves Long-Term Functional Recovery in Rats. J Mol Neurosci 72:730-740 (2022). PubMed: 34988900
- Sahyon HAE et al. Anti-proliferative effects of the combination of Sulfamethoxazole and Quercetin via caspase3 and NFkB gene regulation: an in vitro and in vivo study. Naunyn Schmiedebergs Arch Pharmacol 395:227-246 (2022). PubMed: 34994822