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    active-human-ikb-alpha-phospho-y42-peptide-ab42716.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
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Active human IKB alpha (phospho Y42) peptide (ab42716)

  • Datasheet
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Blocking - Active human IKB alpha (phospho Y42) peptide (ab42716)

    Key features and details

    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: Blocking, ELISA

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    Description

    • Product name

      Active human IKB alpha (phospho Y42) peptide
      See all IKB alpha proteins and peptides
    • Biological activity

      The peptide is specifically recognized by IkBa (Tyr-42) phospho-specific antibody (ab24783) in ELISA, and has been shown to block the reactivity of ab24783 during Western blot. In addition, the peptide is recommended for use in blocking ab24783 reactivity in immunocytochemistry.
    • Purity

      > 90 % SDS-PAGE.

    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human
      • Modifications

        phospho Y42

    Associated products

    • Corresponding Antibody

      • Anti-IKB alpha (phospho Y42) antibody (ab24783)

    Specifications

    Our Abpromise guarantee covers the use of ab42716 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-IKB alpha (phospho Y42) antibody (ab24783)

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Preservative: 0.05% Sodium azide
      Constituent: PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • I kappa B alpha
      • I-kappa-B-alpha
      • IkappaBalpha
      • IkB-alpha
      • IKBA
      • IKBA_HUMAN
      • IKBalpha
      • MAD 3
      • MAD3
      • Major histocompatibility complex enhancer-binding protein MAD3
      • NF kappa B inhibitor alpha
      • NF-kappa-B inhibitor alpha
      • NFKBI
      • NFKBIA
      • Nuclear factor of kappa light chain gene enhancer in B cells
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
      see all
    • Function

      Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
    • Involvement in disease

      Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 5 ANK repeats.
    • Post-translational
      modifications

      Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
      Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
      Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
      Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
    • Target information above from: UniProt accession P25963 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Blocking - Active human IKB alpha (phospho Y42) peptide (ab42716)
      Blocking - Active human IKB alpha (phospho Y42) peptide (ab42716)

      Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IκBα (lane 1), anti-IκBα (Tyr-42) (lanes 2-5), or anti-IκBα (Tyr-305) (lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of ab42716, IκBα (Tyr-42) (lane 3 & 8) or ab42717, IκBα (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab42716? Please let us know so that we can cite the reference in this datasheet.

    ab42716 has not yet been referenced specifically in any publications.

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