Key features and details
- Purity: > 90% HPLC
- Active: Yes
- Suitable for: Blocking, ELISA
Product nameActive human Integrin beta 4 (phospho Y1494) peptide
See all Integrin beta 4 proteins and peptides
Purity> 90 % HPLC.
Reverse phase HPLC, purity analysis is done using HPLC and Mass identification by Mass Spectral Analysis.
Our Abpromise guarantee covers the use of ab42718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CD 104
- CD104 antigen
FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
- Information by UniProt
Western blot analysis of A431 cells untreated (lanes 1, 5, & 9) or treated with pervanadate (1 mM) for 30 mins (lanes 2-4, 6-8, & 10). The blots were probed with anti-Integrin β4 (Tyr-1494), anti-Integrin β4 (Tyr-1526), or anti-Integrin β4 (cytoplasmic) antibody. In some lanes, the antibodies were used in the absence (lanes 2 & 6) or presence of ab42718, Integrin β4 (Tyr-1494) (lanes 3 & 7), or Integrin β4 (Tyr-1526) (lanes 4 & 8) blocking peptides.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab42718 has not yet been referenced specifically in any publications.