Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-YAP1 antibody [EPR19812]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Rat Tissue sections (Kidney)

Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678

Yes - 0.05% tween 20

Kidney

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Formaldehyde

14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block

1/200

1/300

Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Conjugation: Alexa Fl
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

Pseudo colored Green immunofluorescent staining of paraffin embedded rat kidney with purified ab205270 Anti-YAP1 at a working dilution of 1 in 200. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining protocol. The antigen retrieval was performed with the NxGen decloaking chamber at pH6.0 . Alexa Fluor 647 secondary is preferred for its better resolution and total lack of autofluorescence.

We would like to thank the reviewer for submitting this AbReview for ab205270. We are pleased that this antibody worked well for you on rat samples, a species that has not been tested before. We appreciate the time and effort that the reviewer has put into testing this antibody in this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.