Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Conjugation: Alexa Fl
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Pseudo colored Green immunofluorescent staining of paraffin embedded rat kidney with purified ab205270 Anti-YAP1 at a working dilution of 1 in 200. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining protocol. The antigen retrieval was performed with the NxGen decloaking chamber at pH6.0 . Alexa Fluor 647 secondary is preferred for its better resolution and total lack of autofluorescence.
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab205270. We are pleased that this antibody worked well for you on rat samples, a species that has not been tested before. We appreciate the time and effort that the reviewer has put into testing this antibody in this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
Abcam user community
Verified customer
Submitted Feb 13 2020