Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 ab93678
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Conjugation: Alexa Fl
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Pseudo colored Green immunofluorescence staining of paraformaldehyde perfusion fixed frozen pig kidney with purified ab205270 Anti-YAP1 at a working dilution of 1 in 200. The nuclei are Blue after staining with Hoechst and a light Red counter stain was created with a methyl green staining and by using the 555nm excitation filter. Alexa Fluor 647 secondary offers better resolution and it lacks autofluorescence.
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Submitted Jun 29 2020