Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Activin A Receptor Type IB/ALK-4 antibody
See all Activin A Receptor Type IB/ALK-4 primary antibodies -
Description
Rabbit polyclonal to Activin A Receptor Type IB/ALK-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human Activin A Receptor Type IB/ALK-4 (internal sequence). The exact sequence is proprietary.
Database link: P36896 -
Positive control
- WB: SK-N-SH whole cell lysate; mouse brain tissue lysate. IHC: Human colon carcinoma tissue.
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General notes
Previously labelled as Activin A Receptor Type IB.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2. -
Tissue specificity
Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 91 Human
- Entrez Gene: 11479 Mouse
- Entrez Gene: 100152462 Pig
- Entrez Gene: 29381 Rat
- Entrez Gene: 696587 Rhesus monkey
- Entrez Gene: 399454 Xenopus laevis
- Omim: 601300 Human
- SwissProt: P36896 Human
see all -
Form
There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3. -
Alternative names
- Activin A receptor type 1B antibody
- Activin A receptor type II like kinase 4 antibody
- Activin A type 1B receptor antibody
see all
Images
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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433) at 1/3000 dilution + SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa7.5% SDS-PAGE
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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Paraffin-embedded human colon carcinoma tissue stained for Activin A Receptor Type IB/ALK-4 using ab227433 at 1/250 dilution in immunohistochemical analysis.
References
ab227433 has not yet been referenced specifically in any publications.
