Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)
Key features and details
- Rabbit polyclonal to Activin A Receptor Type IB/ALK-4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Activin A Receptor Type IB/ALK-4 antibody
See all Activin A Receptor Type IB/ALK-4 primary antibodies -
Description
Rabbit polyclonal to Activin A Receptor Type IB/ALK-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human Activin A Receptor Type IB/ALK-4 (internal sequence). The exact sequence is proprietary.
Database link: P36896 -
Positive control
- WB: SK-N-SH whole cell lysate; mouse brain tissue lysate. IHC: Human colon carcinoma tissue.
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General notes
Previously labelled as Activin A Receptor Type IB.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2. -
Tissue specificity
Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 91 Human
- Entrez Gene: 11479 Mouse
- Entrez Gene: 100152462 Pig
- Entrez Gene: 29381 Rat
- Entrez Gene: 696587 Rhesus monkey
- Entrez Gene: 399454 Xenopus laevis
- Omim: 601300 Human
- SwissProt: P36896 Human
see all -
Form
There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3. -
Alternative names
- Activin A receptor type 1B antibody
- Activin A receptor type II like kinase 4 antibody
- Activin A type 1B receptor antibody
see all
Images
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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433) at 1/3000 dilution + SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa7.5% SDS-PAGE
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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody (ab227433)Paraffin-embedded human colon carcinoma tissue stained for Activin A Receptor Type IB/ALK-4 using ab227433 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227433 has not yet been referenced specifically in any publications.
