Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4 (Biotin)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Product nameAnti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (Biotin)
See all Activin A Receptor Type IB/ALK-4 primary antibodies
DescriptionRabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4 (Biotin)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Activin A Receptor Type IB/ALK-4 (extracellular). The exact sequence is proprietary.
Database link: P36896
- IHC-P: normal human kidney tissue sections.
Previously labelled as Activin A Receptor Type IB.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab204655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
Tissue specificityExpressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
- Activin A receptor type 1B antibody
- Activin A receptor type II like kinase 4 antibody
- Activin A type 1B receptor antibody
IHC image of Activin A Receptor Type IB/ALK-4 staining in a section of formalin-fixed paraffin-embedded normal human kidney*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab204655 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab204655 has been referenced in 1 publication.
- Janik S et al. Follistatin impacts Tumor Angiogenesis and Outcome in Thymic Epithelial Tumors. Sci Rep 9:17359 (2019). PubMed: 31757999