RecombinantRabMAb

Recombinant Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)

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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
  • Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free
    See all Activin A Receptor Type IB/ALK-4 primary antibodies

  • Description

    Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Activin A Receptor Type IB/ALK-4. The exact sequence is proprietary.

  • General notes

    Ab247825 is the carrier-free version of ab109300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247825 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols. Heat to 98°C, allow to cool for 10-20 minutes.
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.

    • Tissue specificity

      Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.

    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.

    • Alternative names

      • Activin A receptor type 1B antibody
      • Activin A receptor type II like kinase 4 antibody
      • Activin A type 1B receptor antibody
      • Activin A type IB receptor antibody
      • Activin receptor like kinase 4 antibody
      • Activin receptor type 1B antibody
      • Activin receptor type IB antibody
      • Activin receptor type-1B antibody
      • Activin receptor-like kinase 4 antibody
      • ACTR IB antibody
      • ACTR-IB antibody
      • ACTRIB antibody
      • ACV1B_HUMAN antibody
      • ACVR 1B antibody
      • Acvr1b antibody
      • ACVRLK 4 antibody
      • ACVRLK4 antibody
      • ALK 4 antibody
      • ALK-4 antibody
      • ALK4 antibody
      • Serine(threonine) protein kinase receptor R2 antibody
      • Serine/threonine-protein kinase receptor R2 antibody
      • SKR 2 antibody
      • SKR2 antibody
      see all

    Images

    • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (purified) + Human fetal kidney lysate at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab109300, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)

      This data was developed using ab109300, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma sections labelling Activin A Receptor Type IB/ALK-4 with purified ab109300 at dilution of 1/600. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
    • Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)

      This data was developed using ab109300, the same antibody clone in a different buffer formulation.

      Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labelling Activin A Receptor Type IB/ALK-4 with purified ab109300 at 1/250 (5.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594). DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
    • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      This data was developed using ab109300, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (unpurified)

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human fetal kidney tissue lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : Human fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      This data was developed using ab109300, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      This data was developed using ab109300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of human kidney tissue sections labelling Activin A Receptor Type IB/ALK-4 with unpurified ab109300 at 1/500 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Immunocytochemistry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      This data was developed using ab109300, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of 2% paraformaldehyde fixed (20 mins) SH-SY5Y cells labelling Activin A Receptor Type IB/ALK-4 with unpurified ab109300 at a dilution of 1/100. Alexa Fluor488 goat anti-rabbit IgG was used as the secondary antibody.
    • Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)
      Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] - BSA and Azide free (ab247825)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247825? Please let us know so that we can cite the reference in this datasheet.

    ab247825 has not yet been referenced specifically in any publications.

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