Overview

  • Product name

    Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816]
    See all Activin A Receptor Type IB/ALK-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR4816] to Activin A Receptor Type IB/ALK-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Activin A Receptor Type IB/ALK-4 aa 400-500 (intracellular).
    Database link: P36896

  • Positive control

    • HepG2, SH SY5Y and U87 MG cell lysates; 293T cells
  • General notes

    Previously labelled as Activin A Receptor Type IB. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
    • Tissue specificity

      Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Form

      There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
    • Alternative names

      • Activin A receptor type 1B antibody
      • Activin A receptor type II like kinase 4 antibody
      • Activin A type 1B receptor antibody
      • Activin A type IB receptor antibody
      • Activin receptor like kinase 4 antibody
      • Activin receptor type 1B antibody
      • Activin receptor type IB antibody
      • Activin receptor type-1B antibody
      • Activin receptor-like kinase 4 antibody
      • ACTR IB antibody
      • ACTR-IB antibody
      • ACTRIB antibody
      • ACV1B_HUMAN antibody
      • ACVR 1B antibody
      • Acvr1b antibody
      • ACVRLK 4 antibody
      • ACVRLK4 antibody
      • ALK 4 antibody
      • ALK-4 antibody
      • ALK4 antibody
      • Serine(threonine) protein kinase receptor R2 antibody
      • Serine/threonine-protein kinase receptor R2 antibody
      • SKR 2 antibody
      • SKR2 antibody
      see all

    Images

    • All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] (ab133478) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of 293T cells labelled with ab133478 at a 1/10 dilution (red), or negative rabbit IgG (green).

    References

    This product has been referenced in:

    • Hu J  et al. Anesthetic effects of isoflurane and the molecular mechanism underlying isoflurane-inhibited aggressiveness of hepatic carcinoma. Mol Med Rep 18:184-192 (2018). Read more (PubMed: 29749446) »
    • Lau B  et al. The Expression of Human Cytomegalovirus MicroRNA MiR-UL148D during Latent Infection in Primary Myeloid Cells Inhibits Activin A-triggered Secretion of IL-6. Sci Rep 6:31205 (2016). SDS-PAGE . Read more (PubMed: 27491954) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133478.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up