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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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RecombinantRabMAb

Recombinant Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)

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  • Certificate of Compliance
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Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
  • Flow Cytometry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
  • Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4816] to Activin A Receptor Type IB/ALK-4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

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Protein
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Recombinant Human Activin A Receptor Type IB/ALK-4 protein (ab157846)

View more associated products

Overview

  • Product name

    Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free
    See all Activin A Receptor Type IB/ALK-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR4816] to Activin A Receptor Type IB/ALK-4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Activin A Receptor Type IB/ALK-4 aa 400-500 (intracellular). The exact sequence is proprietary.
    Database link: P36896

  • General notes

    Ab248514 is the carrier-free version of ab133478. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248514 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4816
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Surface Molecules

Associated products

  • Alternative Versions

    • Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] (ab133478)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Activin A Receptor Type IB/ALK-4 protein (ab157846)

Applications

Our Abpromise guarantee covers the use of ab248514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
    • Tissue specificity

      Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P36896 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 91 Human
      • Entrez Gene: 11479 Mouse
      • Entrez Gene: 29381 Rat
      • Omim: 601300 Human
      • SwissProt: P36896 Human
      • SwissProt: Q61271 Mouse
      • SwissProt: P80202 Rat
      • Unigene: 438918 Human
      • Unigene: 308467 Mouse
      • Unigene: 214018 Rat
      see all
    • Form

      There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
    • Alternative names

      • Activin A receptor type 1B antibody
      • Activin A receptor type II like kinase 4 antibody
      • Activin A type 1B receptor antibody
      • Activin A type IB receptor antibody
      • Activin receptor like kinase 4 antibody
      • Activin receptor type 1B antibody
      • Activin receptor type IB antibody
      • Activin receptor type-1B antibody
      • Activin receptor-like kinase 4 antibody
      • ACTR IB antibody
      • ACTR-IB antibody
      • ACTRIB antibody
      • ACV1B_HUMAN antibody
      • ACVR 1B antibody
      • Acvr1b antibody
      • ACVRLK 4 antibody
      • ACVRLK4 antibody
      • ALK 4 antibody
      • ALK-4 antibody
      • ALK4 antibody
      • Serine(threonine) protein kinase receptor R2 antibody
      • Serine/threonine-protein kinase receptor R2 antibody
      • SKR 2 antibody
      • SKR2 antibody
      see all

    Images

    • Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
      Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
      All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] (ab133478) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab133478, the same antibody clone in a different buffer formulation.

    • Flow Cytometry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
      Flow Cytometry - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
      This data was developed using ab133478, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 293T cells labelled with ab133478 at a 1/10 dilution (red), or negative rabbit IgG (green).
    • Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)
      Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4816] - BSA and Azide free (ab248514)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab248514? Please let us know so that we can cite the reference in this datasheet.

    ab248514 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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