Recombinant
RabMAb

Anti-Activin Receptor Type IA antibody [EPR4076(2)] (ab155981)

Overview

  • Product name
    Anti-Activin Receptor Type IA antibody [EPR4076(2)]
    See all Activin Receptor Type IA primary antibodies
  • Description
    Rabbit monoclonal [EPR4076(2)] to Activin Receptor Type IA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Activin Receptor Type IA aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q04771

  • Positive control
    • WB: human, rat and mouse fetal brain lysate IP: mouse brain lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IP 1/10 - 1/100.

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis.
  • Tissue specificity
    Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells.
  • Involvement in disease
    Defects in ACVR1 are a cause of fibrodysplasia ossificans progressiva (FOP) [MIM:135100]. FOP is a rare autosomal dominant disorder of skeletal malformations and progressive extraskeletal ossification. Heterotopic ossification in FOP begins in childhood and can be induced by trauma or may occur without warning. Bone formation is episodic and progressive, leading to extra-articular ankylosis of all major joints of the axial and appendicular skeleton, rendering movement impossible.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin A receptor type I antibody
    • Activin A receptor type II like kinase 2 antibody
    • Activin receptor type I antibody
    • Activin receptor type-1 antibody
    • Activin receptor-like kinase 2 antibody
    • ACTR-I antibody
    • ACTRI antibody
    • Acvr1 antibody
    • ACVR1_HUMAN antibody
    • ACVR1A antibody
    • ACVRLK2 antibody
    • ALK 2 antibody
    • ALK-2 antibody
    • ALK2 antibody
    • FOP antibody
    • Hydroxyalkyl protein kinase antibody
    • Serine/threonine-protein kinase receptor R1 antibody
    • SKR1 antibody
    • TGF-B superfamily receptor type I antibody
    • TGFB superfamily receptor type I antibody
    • TSR-I antibody
    • TSRI antibody
    see all

Images

  • ab155981 (purified) at 1:50 dilution (2µg) immunoprecipitating Activin Receptor Type IA in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab155981 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155981 in Mouse brain lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • All lanes : Anti-Activin Receptor Type IA antibody [EPR4076(2)] (ab155981) at 0.5 µg/ml (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-Activin Receptor Type IA antibody [EPR4076(2)] (ab155981) at 0.5 µg/ml (purified) + Human fetal brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Activin Receptor Type IA antibody [EPR4076(2)] (ab155981) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal heart lysate
    Lane 3 : Fetal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

References

This product has been referenced in:
  • Rigueur D  et al. The type I BMP receptor ACVR1/ALK2 is required for chondrogenesis during development. J Bone Miner Res 30:733-41 (2015). Read more (PubMed: 25413979) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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