Overview

  • Product name
    Anti-Activin Receptor Type IIA antibody [149/1]
    See all Activin Receptor Type IIA primary antibodies
  • Description
    Mouse monoclonal [149/1] to Activin Receptor Type IIA
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant intracellular kinase domain of the human type 2 activin A receptor (ACVR2A).

Properties

Applications

Our Abpromise guarantee covers the use of ab18103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin A receptor type IIA antibody
    • Activin receptor type 2A antibody
    • Activin receptor type IIA antibody
    • Activin receptor type-2A antibody
    • ACTR 2 antibody
    • ACTR IIA antibody
    • ACTR-IIA antibody
    • ACTR2 antibody
    • ACTRII antibody
    • ACTRIIA antibody
    • Acvr 2 antibody
    • Acvr 2A antibody
    • Acvr2 antibody
    • ACVR2A antibody
    • AVR2A_HUMAN antibody
    • OTTHUMP00000197918 antibody
    see all

References

This product has been referenced in:
  • McCarthy SA  et al. Monoclonal antibodies that recognize the type-2 activin receptor, ACTR2. Hybridoma 13:199-203 (1994). Read more (PubMed: 7927363) »
  • McCarthy SA & Bicknell R Activin-A binds to a heterotrimeric receptor complex on the vascular endothelial cell surface. Evidence for a type 3 activin receptor. J Biol Chem 269:3909-12 (1994). Read more (PubMed: 8307945) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Recombinant protein (HEK293T cells)
Loading amount
5e+006 cells
Specification
HEK293T cells
Treatment
transfected with cytoplasmic domain of mouse Acvr2b in pDEST26 vector (expected size: 387 amino acids)
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Teresa Gunn

Verified customer

Submitted Aug 08 2007

Answer

Thanks for your email and I'm sorry to hear about the bug in your customer's vial. I do apologize, and it is very strange. We will send a vial of ab18103 as a free of charge replacement on your next shipment. Please let me know if you have any additional questions or concerns. Have a great weekend!

Read More

Answer

Thank you for your enquiry. The following two references provide more information regarding this antibody: McCarthy SA et al. Monoclonal antibodies that recognize the type-2 activin receptor, ACTR2. Hybridoma 13:199-203 (1994). PubMed: 7927363 McCarthy SA & Bicknell R Activin-A binds to a heterotrimeric receptor complex on the vascular endothelial cell surface. Evidence for a type 3 activin receptor. J Biol Chem 269:3909-12 (1994). PubMed: 8307945 The Hybridoma reference states that the antibody recognizes the kinase domain of Activin Receptor 2 but does not mention subtypes a or b. The portion of Activin Receptor II that is used for blots in this paper is the 802bp Ssp I fragment of human ACTR2 cDNA. The fragment's isolation is described in McCarthy SA et al. 1993. J Biol Chem. 268: 23066-71 which may be of interest to you. I hope this is helpful. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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