Overview

  • Product name

    Anti-Activin Receptor Type IIA/ACVR2A antibody
    See all Activin Receptor Type IIA/ACVR2A primary antibodies
  • Description

    Rabbit polyclonal to Activin Receptor Type IIA/ACVR2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Activin Receptor Type IIA/ACVR2A aa 2-29 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: 92

  • Positive control

    • Mouse liver, brain and kidney tissue lysates. 293 cells lysate and Human hepatocarcinoma tissue.
  • General notes

     This product was previously labelled as Activin Receptor Type IIA

     

Properties

Applications

Our Abpromise guarantee covers the use of ab135634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activin A receptor type IIA antibody
    • Activin receptor type 2A antibody
    • Activin receptor type IIA antibody
    • Activin receptor type-2A antibody
    • ACTR 2 antibody
    • ACTR IIA antibody
    • ACTR-IIA antibody
    • ACTR2 antibody
    • ACTRII antibody
    • ACTRIIA antibody
    • Acvr 2 antibody
    • Acvr 2A antibody
    • Acvr2 antibody
    • ACVR2A antibody
    • AVR2A_HUMAN antibody
    • OTTHUMP00000197918 antibody
    see all

Images

  • All lanes : Anti-Activin Receptor Type IIA/ACVR2A antibody (ab135634) at 1/100 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 58 kDa

  • All lanes : Anti-Activin Receptor Type IIA/ACVR2A antibody (ab135634) at 1/100 dilution

    Lane 1 : 293 cell lysates nontransfected
    Lane 2 : 293 cell lysates transiently transfected with the Activin Receptor Type IIA/ACVR2A gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 58 kDa

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human hepatocarcinoma tissue labelling Activin Receptor Type IIA/ACVR2A with ab135634 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.

References

This product has been referenced in:

  • Zhang H  et al. Adenovirus-mediated knockdown of activin A receptor type 2A attenuates immune-induced hepatic fibrosis in mice and inhibits interleukin-17-induced activation of primary hepatic stellate cells. Int J Mol Med 42:279-289 (2018). Read more (PubMed: 29620144) »
  • Dillenburg A  et al. Activin receptors regulate the oligodendrocyte lineage in health and disease. Acta Neuropathol 135:887-906 (2018). Read more (PubMed: 29397421) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Testis Lysate)
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Loading amount
40 µg
Specification
Mouse Testis Lysate
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Dec 04 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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