Overview

  • Product name
    Anti-Activin Receptor Type IIA/ACVR2A antibody
    See all Activin Receptor Type IIA/ACVR2A primary antibodies
  • Description
    Rabbit polyclonal to Activin Receptor Type IIA/ACVR2A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human Activin Receptor Type IIA/ACVR2A aa 267-509. (AAH69707)

  • Positive control
    • HeLa or HepG2 whole cell lysate (ab7900) This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: DU145.
  • General notes

     This product was previously labelled as Activin Receptor Type IIA

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 58 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-Activin Receptor Type IIA/ACVR2A antibody (ab96793) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • ICC/IF image of ab96793 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96793 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • O'Keeffe GW  et al. Region-specific role of growth differentiation factor-5 in the establishment of sympathetic innervation. Neural Dev 11:4 (2016). ICC . Read more (PubMed: 26878848) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for calling Abcam earlier today.

As we discussed, I am sending you ab18130 free of charge so that you can confirm the western blot results that you are seeing with ab96793.

If there is anything else I can help you with, please let me know.

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