Overview

  • Product name

    Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739]
    See all Activin Receptor Type IIB/ACVR2B primary antibodies
  • Description

    Rabbit monoclonal [EPR10739] to Activin Receptor Type IIB/ACVR2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Activin Receptor Type IIB/ACVR2B aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13705

  • Positive control

    • WB: K562, NIH:OVCAR-3, 293T and Jurkat whole cell lysate (ab7899), mouse and rat heart tissue lysates. IP: HEK-283T whole cell lysate.
  • General notes

     This product was previously labelled as Activin Receptor Type IIB

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
    • Involvement in disease

      Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activin A receptor type IIB antibody
      • Activin receptor type 2B antibody
      • Activin receptor type IIB antibody
      • Activin receptor type-2B antibody
      • ActR IIB antibody
      • ACTR-IIB antibody
      • ActRIIB antibody
      • ACVR 2B antibody
      • ACVR2B antibody
      • AVR2B_HUMAN antibody
      • HTX4 antibody
      • MGC116908 antibody
      see all

    Images

    • Activin Receptor Type IIB/ACVR2B was immunoprecipitated from 0.35 mg HEK-293T ( Human embryonic kidney epithelial cell) whole cell lysate with ab180185 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab180185 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
      Lane 1: HEK-293T whole cell lysate 10 µg (Input).
      Lane 2: ab180185 IP in HEK-283T whole cell lysate (+).
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180185 in HEK-293T whole cell lysate (-).
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/5000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 58 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/5000 dilution (purified)

      Lane 1 : K562 whole cell lysate
      Lane 2 : NIH:OVCAR-3 whole cell lysate
      Lane 3 : HEK293 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 58 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [EPR10739] (ab180185) at 1/1000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : NIH:OVCAR-3 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 58 kDa

    References

    This product has been referenced in:

    • Yang J  et al. Effect of exercise on bone in poorly controlled type 1 diabetes mediated by the ActRIIB/Smad signaling pathway. Exp Ther Med 16:3686-3693 (2018). Read more (PubMed: 30233727) »
    • Tang L  et al. Low-intensity pulsed ultrasound prevents muscle atrophy induced by type 1 diabetes in rats. Skelet Muscle 7:29 (2017). Read more (PubMed: 29273088) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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