Overview

  • Product name

    Anti-ACTL6A antibody [EPR7443]
    See all ACTL6A primary antibodies
  • Description

    Rabbit monoclonal [EPR7443] to ACTL6A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ACTL6A (O96019).

  • Positive control

    • Ramos, K562, HeLa and Jurkat whole cell lysate (ab7899); Human breast carcinoma and colon tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 53 kDa BRG1 associated factor A antibody
      • 53 kDa BRG1-associated factor A antibody
      • ACL6A_HUMAN antibody
      • Actin like 6A antibody
      • Actin like protein 6A antibody
      • Actin related protein 4 antibody
      • Actin related protein antibody
      • Actin related protein Baf53a antibody
      • Actin-like protein 6A antibody
      • Actin-related protein Baf53a antibody
      • ACTL 6 antibody
      • ACTL 6A antibody
      • ACTL6 antibody
      • ACTL6A antibody
      • Arp4 antibody
      • ARPN BETA antibody
      • ArpNbeta antibody
      • BAF 53A antibody
      • BAF complex 53 kDa subunit antibody
      • BAF53 antibody
      • BAF53A antibody
      • BRG1 associated factor antibody
      • BRG1-associated factor 53A antibody
      • hArpN beta antibody
      • INO80 complex subunit K antibody
      • INO80K antibody
      • MGC5382 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ACTL6A with ab131272 at 1/50 dilution.
    • All lanes : Anti-ACTL6A antibody [EPR7443] (ab131272) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling ACTL6A with ab131272 at 1/50 dilution.

    References

    This product has been referenced in:

    • Laurette P  et al. Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells. Elife 4:N/A (2015). WB ; Human . Read more (PubMed: 25803486) »
    • Pfister NT  et al. Mutant p53 cooperates with the SWI/SNF chromatin remodeling complex to regulate VEGFR2 in breast cancer cells. Genes Dev 29:1298-315 (2015). Human . Read more (PubMed: 26080815) »
    See all 2 Publications for this product

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