Key features and details
- Rabbit polyclonal to ACTL7B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACTL7B antibody
DescriptionRabbit polyclonal to ACTL7B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: HEK-293T overexpressing ACTL7B cell lysate. IHC-P: Human testis tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 45 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityDetected only in the testis and, to a lesser extent, in the prostate.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ACL7B_HUMAN antibody
- Actin like 7 beta antibody
- Actin like 7B antibody
Paraffin-embedded human testis tissue stained for ACTL7B using ab223716 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-ACTL7B antibody (ab223716) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate (negative control)
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing ACTL7B (co-expressed with a c-terminal myc-DDK tag), cell lysate
Predicted band size: 45 kDa
ab223716 has not yet been referenced specifically in any publications.