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Rabbit polyclonal to ACTL8
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 156 and 353 of ACTL8 (NP_110439)
- A431 and Molt-4 whole cell lysate. H1299, HeLa, HepG2 cell lines. A549 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1.21% Tris, 0.75% Glycine
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500.
||1/500 - 1/3000. Predicted molecular weight: 41 kDa.
||1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.
Strongly expressed in testis and pancreas. Weak expression in placenta.
Belongs to the actin family.
Cytoplasm > cytoskeleton.
Information by UniProt
- Actin like 8 antibody
- Actin like protein 8 antibody
- Actin like protein antibody
Western blot - Anti-ACTL8 antibody (ab96756)
All lanes : Anti-ACTL8 antibody (ab96756) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL8 antibody (ab96756)
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ACTL8 antibody (ab96756)
ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.
has not yet been referenced specifically in any publications.
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