Key features and details
- Rabbit polyclonal to ACTL8
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACTL8 antibody
See all ACTL8 primary antibodies
DescriptionRabbit polyclonal to ACTL8
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 156 and 353 of ACTL8 (NP_110439)
- A431 and Molt-4 whole cell lysate. H1299, HeLa, HepG2 cell lines. A549 xenograft tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.|
Tissue specificityStrongly expressed in testis and pancreas. Weak expression in placenta.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Actin like 8 antibody
- Actin like protein 8 antibody
- Actin like protein antibody
All lanes : Anti-ACTL8 antibody (ab96756) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.
ab96756 has not yet been referenced specifically in any publications.