Key features and details
- Rabbit polyclonal to ACTL9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ACTL9 antibody
DescriptionRabbit polyclonal to ACTL9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- ICC/IF: HepG2 and MCF7 cells. IHC-P: Human kidney tissue. WB: Mouse heart and skeletal muscle tissue lysates.
This product was previously labelled as MGC33407
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 35,46 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- actin-like 9 antibody
- Actin-like protein 9 antibody
- ACTL7C antibody
All lanes : Anti-ACTL9 antibody (ab229876) at 1/2000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 35,46 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ACTL9 using ab229876 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
MCF7 (human breast adenocarcinoma cell line) cells stained for ACTL9 using ab229876 at 1/100 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human kidney tissue stained for ACTL9 using ab229876 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229876 has not yet been referenced specifically in any publications.