Overview

  • Product name

  • Description

    Rabbit polyclonal to ACTN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish ACTN3 aa 16-378. The exact sequence is proprietary.
    Database link: NP_571597.1

  • Positive control

    • Whole Zebrafish whole cell lysate; Zebrafish embryo.

Properties

Applications

Our Abpromise guarantee covers the use of ab211127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
IHC - Wholemount 1/100 - 1/500.

Target

  • Function

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity

    Expressed only in a subset of type 2 skeletal muscle fibers.
  • Sequence similarities

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Information by UniProt
  • Database links

    • Alternative names

      • Actinin alpha 3 antibody
      • actinin, alpha antibody
      • ACTN 3 antibody
      • ACTN3 antibody
      • ACTN3_HUMAN antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 3 antibody
      • Alpha-Actinin 3 antibody
      • Alpha-actinin skeletal muscle isoform 3 antibody
      • Alpha-actinin-3 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • MGC117002 antibody
      • MGC117005 antibody
      • OTTHUMP00000219049 antibody
      • OTTHUMP00000219054 antibody
      see all

    Images

    • Immunohistochemical analysis of paraformaldehyde-fixed Zebrafish embryo labeling ACTN3 with ab211127 at 1/200 dilution.

    • Anti-ACTN3 antibody (ab211127) at 1/1000 dilution + Whole Zebrafish whole cell lysate at 30 µg

      Predicted band size: 103 kDa



      7.5% SDS PAGE

    References

    ab211127 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    IHC - Wholemount
    Sample
    Xenopus laevis Embryo (dorsal embryo)
    Specification
    dorsal embryo

    Abcam user community

    Verified customer

    Submitted May 09 2017

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