Recombinant
RabMAb

Recombinant Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833)

Overview

  • Product name

    Anti-ACTR1B + ACTR1A antibody [EPR16968(B)]
    See all ACTR1B + ACTR1A primary antibodies
  • Description

    Rabbit monoclonal [EPR16968(B)] to ACTR1B + ACTR1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ACTR1B + ACTR1A aa 50-150. The exact sequence is proprietary.
    Database link: P42025

  • Positive control

    • WB: Jurkat, MOLT-4 and HeLa whole cell lysates; Human fetal brain and fetal kidney lysates; Full length Human ACTR1A recombination protein. IP: Jurkat whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16968(B)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab203833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IP 1/40.

Target

  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Actin related protein 1B antibody
    • Actin RPV antibody
    • Alpha centractin antibody
    • ARP1 actin related protein 1 homolog A centractin alpha antibody
    • ARP1 actin related protein 1 homolog B centractin beta antibody
    • ARP1 antibody
    • Arp1A antibody
    • ARP1B antibody
    • Beta centractin antibody
    • Centractin antibody
    • Centractin beta antibody
    • Centrosome associated actin homolog antibody
    • CTRN1 antibody
    • CTRN2 antibody
    • PC3 antibody
    see all

Images

  • All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution + Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 second
  • ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500.

    Lane 1: Jurkat whole cell lysate 10ug (Input). Lane 2: ab203833 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203833 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab203833 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203833.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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