Recombinant Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
![Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)](https://www.abcam.com/ps/products/251/ab251404/Images/ab251404-408250-anti-actr1b-actr1a-antibody-epr16968-b-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16968(B)] to ACTR1B + ACTR1A - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free
See all ACTR1B + ACTR1A primary antibodies -
Description
Rabbit monoclonal [EPR16968(B)] to ACTR1B + ACTR1A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251404 is the carrier-free version of ab203833. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab251404 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16968(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
|
IP
Use at an assay dependent concentration. |
Target
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Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10120 Human
- Entrez Gene: 10121 Human
- Omim: 605143 Human
- Omim: 605144 Human
- SwissProt: P42025 Human
- SwissProt: P61163 Human
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Alternative names
- Actin related protein 1B antibody
- Actin RPV antibody
- Alpha centractin antibody
see all
Images
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Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab203833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)](https://www.abcam.com/ps/products/251/ab251404/Images/ab251404-406008-anti-actr1b-actr1a-antibody-epr16968-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)All lanes : Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab203833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)](https://www.abcam.com/ps/products/251/ab251404/Images/ab251404-403179-anti-actr1b-actr1a-antibody-epr16968-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] (ab203833) at 1/1000 dilution + Full length Human ACTR1A recombination protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 secondThis data was developed using ab203833, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)](https://www.abcam.com/ps/products/251/ab251404/Images/ab251404-1-anti-actr1b-actr1a-antibody-epr16968-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)This data was developed using ab203833, the same antibody clone in a different buffer formulation.ACTR1B + ACTR1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab203833 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203833 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500. Lane 1: Jurkat whole cell lysate 10ug (Input). Lane 2: ab203833 IP in Jurkat whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203833 in Jurkat whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. -
![Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)](https://www.abcam.com/ps/products/251/ab251404/Images/ab251404-5-benefits-of-recombinant-antibodies.png)
Anti-ACTR1B + ACTR1A antibody [EPR16968(B)] - BSA and Azide free (ab251404)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab251404 has not yet been referenced specifically in any publications.