Anti-AcV5 tag antibody [AcV5] (ab49581)
Key features and details
- Mouse monoclonal [AcV5] to AcV5 tag
- Suitable for: IP, ICC, Dot blot
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-AcV5 tag antibody [AcV5] -
Description
Mouse monoclonal [AcV5] to AcV5 tag -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC, Dot blotmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Tissue, cells or virus corresponding to AcV5 tag. AcNPV extracellular non occluded virus (NOV)
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Positive control
- Extracts of Sf9 cells infected with baculovirus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
AcV5 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent dilution.
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| ICC |
Use at an assay dependent dilution.
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| Dot blot |
Use a concentration of 0.5 - 1 µg/ml.
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| Notes |
|---|
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IP
Use at an assay dependent dilution. |
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ICC
Use at an assay dependent dilution. |
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Dot blot
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Relevance
Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab49581 has been referenced in 1 publication.
- Ermakova M et al. Installation of C4 photosynthetic pathway enzymes in rice using a single construct. Plant Biotechnol J 19:575-588 (2021). PubMed: 33016576