Anti-AcV5 tag antibody [AcV5] (ab49581)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-AcV5 tag antibody [AcV5]
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DescriptionMouse monoclonal [AcV5] to AcV5 tag
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Host speciesMouse
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Tested applicationsSuitable for: IP, ICC, Dot blotmore details
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Species reactivityReacts with: Species independent
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Immunogen
AcNPV extracellular nonocculeded virus (NOV)
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Positive control
- Extracts of Sf9 cells infected with baculovirus.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
ClonalityMonoclonal
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Clone numberAcV5
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MyelomaSp2/0-Ag14
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab49581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent dilution. | |
| ICC | Use at an assay dependent dilution. | |
| Dot blot | Use a concentration of 0.5 - 1 µg/ml. |
Target
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RelevanceEpitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.
References
ab49581 has not yet been referenced specifically in any publications.
