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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-ACY-1 antibody [EPR8445] (ab133635)

  • Datasheet
  • SDS
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Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635)
  • OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (ab133635)
  • Anti-ACY-1 antibody [EPR8445] (ab133635)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8445] to ACY-1
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human ACY-1 protein (His tag) (ab203546)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ACY-1 antibody [EPR8445]
    See all ACY-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8445] to ACY-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ACY-1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • K562 cell lysate, HeLa cell lysate, HepG2 cell lysate
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8445
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-ACY-1 antibody [EPR8445] - BSA and Azide free (ab248584)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ACY-1 protein (His tag) (ab203546)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).
    • Tissue specificity

      Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
    • Involvement in disease

      Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.
    • Sequence similarities

      Belongs to the peptidase M20A family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q03154 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 95 Human
      • Entrez Gene: 109652 Mouse
      • Entrez Gene: 300981 Rat
      • Omim: 104620 Human
      • SwissProt: Q03154 Human
      • SwissProt: Q99JW2 Mouse
      • SwissProt: Q6AYS7 Rat
      • Unigene: 334707 Human
      • Unigene: 7165 Mouse
      • Unigene: 3679 Rat
      see all
    • Alternative names

      • ACY 1 antibody
      • ACY-1 antibody
      • Acy1 antibody
      • ACY1_HUMAN antibody
      • ACY1D antibody
      • ACYLASE antibody
      • Acylase I antibody
      • Aminoacylase 1 antibody
      • Aminoacylase-1 antibody
      • EC 3.5.1.14 antibody
      • epididymis secretory protein Li 5 antibody
      • HEL-S-5 antibody
      • N acyl L amino acid amidohydrolase antibody
      • N-acyl-L-amino-acid amidohydrolase antibody
      • OTTHUMP00000212459 antibody
      • OTTHUMP00000212462 antibody
      • OTTHUMP00000212463 antibody
      • OTTHUMP00000212464 antibody
      • OTTHUMP00000212465 antibody
      see all

    Images

    • Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635)
      Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635)
      All lanes : Anti-ACY-1 antibody [EPR8445] (ab133635) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 46 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?

    • OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (ab133635)
      OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (ab133635)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-ACY-1 antibody [EPR8445] (ab133635)
      Anti-ACY-1 antibody [EPR8445] (ab133635)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab133635? Please let us know so that we can cite the reference in this datasheet.

    ab133635 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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