Overview

  • Product name

    Anti-ACY-1 antibody [EPR8445]
    See all ACY-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8445] to ACY-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACY-1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • K562 cell lysate, HeLa cell lysate, HepG2 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).
    • Tissue specificity

      Expression is highest in kidney, strong in brain and weaker in placenta and spleen.
    • Involvement in disease

      Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.
    • Sequence similarities

      Belongs to the peptidase M20A family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACY 1 antibody
      • ACY-1 antibody
      • Acy1 antibody
      • ACY1_HUMAN antibody
      • ACY1D antibody
      • ACYLASE antibody
      • Acylase I antibody
      • Aminoacylase 1 antibody
      • Aminoacylase-1 antibody
      • EC 3.5.1.14 antibody
      • epididymis secretory protein Li 5 antibody
      • HEL-S-5 antibody
      • N acyl L amino acid amidohydrolase antibody
      • N-acyl-L-amino-acid amidohydrolase antibody
      • OTTHUMP00000212459 antibody
      • OTTHUMP00000212462 antibody
      • OTTHUMP00000212463 antibody
      • OTTHUMP00000212464 antibody
      • OTTHUMP00000212465 antibody
      see all

    Images

    • All lanes : Anti-ACY-1 antibody [EPR8445] (ab133635) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 46 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133635 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133635.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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