Recombinant
Recombinant Anti-ACY-1 antibody [EPR8445] (ab133635)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ACY-1 antibody [EPR8445]
See all ACY-1 primary antibodies -
Description
Rabbit monoclonal [EPR8445] to ACY-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ACY-1 aa 350-450. The exact sequence is proprietary.
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Positive control
- K562 cell lysate, HeLa cell lysate, HepG2 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8445 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa). | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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Function
Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). -
Tissue specificity
Expression is highest in kidney, strong in brain and weaker in placenta and spleen. -
Involvement in disease
Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. -
Sequence similarities
Belongs to the peptidase M20A family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 95 Human
- Entrez Gene: 109652 Mouse
- Entrez Gene: 300981 Rat
- Omim: 104620 Human
- SwissProt: Q03154 Human
- SwissProt: Q99JW2 Mouse
- SwissProt: Q6AYS7 Rat
- Unigene: 334707 Human
see all -
Alternative names
- ACY 1 antibody
- ACY-1 antibody
- Acy1 antibody
see all
Images
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![Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635)](https://www.abcam.com/ps/products/133/ab133635/Images/ab133635-147542-anti-acy-1-antibody-epr8445-western-blot.jpg)
Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635)All lanes : Anti-ACY-1 antibody [EPR8445] (ab133635) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
![Other - Anti-ACY-1 antibody [EPR8445] (ab133635)](https://www.abcam.com/ps/products/133/ab133635/Images/ab133635-147541-anti-acy-1-antibody-epr8445.jpg)
Other - Anti-ACY-1 antibody [EPR8445] (ab133635)
Datasheets and documents
References
ab133635 has not yet been referenced specifically in any publications.
