Overview

  • Product name

    Anti-acyl-CoA Thioesterase 2 antibody
    See all acyl-CoA Thioesterase 2 primary antibodies
  • Description

    Rabbit polyclonal to acyl-CoA Thioesterase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide designed within residues: SPLEGPDQKS FIPVERAEST FLFLVGQDDH NWKSEFYANE ACKRLQAHGR, corresponding to internal sequence amino acids 360-409 of Human acyl-CoA Thioesterase 2 (NP_006812)

  • Positive control

    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    Acyl-CoA Thioesterase 2, also known as ACOT2, is a member of the acyl coenzyme family. Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. There are two isoforms produced by alternative splicing.
  • Cellular localization

    Mitochondrial
  • Database links

  • Alternative names

    • AA571646 antibody
    • ACOT2 antibody
    • Acyl CoA thioesterase 2 antibody
    • Acyl coenzyme A thioester hydrolase 2a antibody
    • Acyl coenzyme A thioesterase 2, mitochondrial antibody
    • CTE Ia antibody
    • CTE1A antibody
    • Long chain acyl CoA thioesterase 2 antibody
    • Mitochondrial acyl CoA thioesterase 1 antibody
    • Mitochondrial acyl CoA thioesterase 2 antibody
    • MTE1 antibody
    • Peroxisomal long chain acyl coA antibody
    • PTE2 antibody
    • PTE2A antibody
    • ZAP128 antibody
    see all

Images

  • Anti-acyl-CoA Thioesterase 2 antibody (ab84644) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size: 53 kDa

References

ab84644 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (rat heart)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
25 µg
Specification
rat heart
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 04 2015

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab84644 for mouse reactivity. (There is only one discount allowed per purchase, so if you wanted to receive a discount for testing rat reactivity, you would need to contact us first to receive another code and then purchase another vial of ab84644). This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview of your mouse data and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. We publish both positive and negative Abreviews on our datasheets so please submit the results of your test regardless of your results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up