Anti-Acyloxyacyl Hydrolase antibody (ab222913)
Key features and details
- Rabbit polyclonal to Acyloxyacyl Hydrolase
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Acyloxyacyl Hydrolase antibody
See all Acyloxyacyl Hydrolase primary antibodies -
Description
Rabbit polyclonal to Acyloxyacyl Hydrolase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Acyloxyacyl Hydrolase aa 180-254.
Database link: P28039 -
Positive control
- WB: A549 whole cell lysate; Mouse skeletal muscle tissue lysate; Rat lung tissue lysate. IHC-P: Human pancreatic cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 62,66 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 62,66 kDa. |
Target
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Function
Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. -
Sequence similarities
Contains 1 saposin B-type domain. -
Post-translational
modificationsBoth subunits contain a number of cysteine residues that may form disulfide bridges. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 313 Human
- Entrez Gene: 27052 Mouse
- Entrez Gene: 498757 Rat
- Omim: 102593 Human
- SwissProt: P28039 Human
- SwissProt: O35298 Mouse
- Unigene: 488007 Human
- Unigene: 314046 Mouse
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Alternative names
- Acyloxyacyl hydrolase (neutrophil) antibody
- Acyloxyacyl hydrolase large subunit antibody
- AOAH antibody
- AOAH_HUMAN antibody
Images
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Paraffin-embedded human pancreatic cancer tissue stained for Acyloxyacyl Hydrolase using ab222913 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-Acyloxyacyl Hydrolase antibody (ab222913) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat lung tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 62,66 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222913 has not yet been referenced specifically in any publications.