Anti-Acyloxyacyl Hydrolase antibody (ab233405)
Key features and details
- Rabbit polyclonal to Acyloxyacyl Hydrolase
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Acyloxyacyl Hydrolase antibody
See all Acyloxyacyl Hydrolase primary antibodies -
Description
Rabbit polyclonal to Acyloxyacyl Hydrolase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Acyloxyacyl Hydrolase aa 1-300. (Expressed in E.coli).
Database link: P28039 -
Positive control
- IHC-P: Human appendix, breast cancer, prostate cancer, glioma, liver and stomach tissues. WB: Human serum; Pig stomach, small intestine and spleen lysates; Recombinant human Acyloxyacyl Hydrolase protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233405 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 20 µg/ml. Predicted molecular weight: 65 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 20 µg/ml. Predicted molecular weight: 65 kDa. |
Target
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Function
Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. -
Sequence similarities
Contains 1 saposin B-type domain. -
Post-translational
modificationsBoth subunits contain a number of cysteine residues that may form disulfide bridges. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 313 Human
- Entrez Gene: 100522290 Pig
- Omim: 102593 Human
- SwissProt: P28039 Human
- Unigene: 488007 Human
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Alternative names
- Acyloxyacyl hydrolase (neutrophil) antibody
- Acyloxyacyl hydrolase large subunit antibody
- AOAH antibody
- AOAH_HUMAN antibody
Images
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Paraffin-embedded human appendix tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human breast cancer tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human prostate cancer tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human glioma tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/ml + Recombinant human Acyloxyacyl Hydrolase protein
Predicted band size: 65 kDa -
Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/ml + Pig stomach lysate
Predicted band size: 65 kDa -
Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/ml + Human serum
Predicted band size: 65 kDa -
Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/ml + Pig small intestine lysate
Predicted band size: 65 kDa -
Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/ml + Pig spleen lysate
Predicted band size: 65 kDa -
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233405 has not yet been referenced specifically in any publications.