Overview

  • Product name
    Anti-ADA antibody (Biotin)
    See all ADA primary antibodies
  • Description
    Rabbit polyclonal to ADA (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cyt, Dot blot, ELISA, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Adenosine Deaminase from Calf spleen.

  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab34677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

(PMID 20147632)

 

 

 

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

Immunomicroscopy Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
  • Tissue specificity
    Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
  • Involvement in disease
    Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
  • Sequence similarities
    Belongs to the adenosine and AMP deaminases family.
  • Cellular localization
    Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ada antibody
    • ADA_HUMAN antibody
    • ADA1 antibody
    • Adenosine aminohydrolase antibody
    • Adenosine deaminase antibody
    see all

References

This product has been referenced in:
  • Soslow JH  et al. Increased Number of Circulating CD8/CD26 T Cells in the Blood of Duchenne Muscular Dystrophy Patients Is Associated with Augmented Binding of Adenosine Deaminase and Higher Muscular Strength Scores. Front Pharmacol 8:914 (2017). Read more (PubMed: 29326589) »
  • Chou JP  et al. Accelerated aging in HIV/AIDS: novel biomarkers of senescent human CD8+ T cells. PLoS One 8:e64702 (2013). Flow Cyt ; Human . Read more (PubMed: 23717651) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products. According to the datasheets, ab54969 has not been tested for ELISA (only WB, Flow Cytmetry); whilst the other antibody (ab34677) has been characterized for indirect ELISA system. Unfortunately, these two antibodies have not been used as a pair in Sandwich ELISA (sELISA). I have conducted a search for you and though we have 8 antibodies against this target (ADA) in our catalogue, none have been tested specifically for sELISA. Apologies! If you need anything further or any help then please let me know.  

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Answer

Thank you for your patience while I investigated the source of the note on our datasheet that specified the molecular weight of ADA as 104 kDa. The predicted molecular weight should be given as 40.7 kDa, based on the UniProt database entry: http://www.uniprot.org/uniprot/P00813 The notation that specified the molecular weight as 104 kDa appears to have been a mistake and was not based on data. What I found when investigating the source of the note was that the antibody had not in fact been tested in western blotting. The application has been removed from the datasheet. It is possible the antibody will stain a western blot effectively, but we do not currently guarantee this. I apologize for any inconvenience. Although we do not have another biotinylated antibody against ADA, we have several unconjugated anti-ADA antibodies, including the rabbit monoclonal ab108352, which recognizes a protein at the predicted molecular weight for ADA. https://www.abcam.com/index.html?datasheet=108352

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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