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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Deamination
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RecombinantRabMAb

Recombinant Anti-ADA antibody [EPR4438] (ab108352)

  • Datasheet
  • SDS
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Western blot - Anti-ADA antibody [EPR4438] (ab108352)
  • Anti-ADA antibody [EPR4438] (ab108352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4438] to ADA
  • Suitable for: WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-ADA antibody [EPR4438]
    See all ADA primary antibodies
  • Description

    Rabbit monoclonal [EPR4438] to ADA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, fetal thymus, and rat kidney lysates
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4438
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Deamination
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-ADA antibody [EPR4438] - BSA and Azide free (ab247642)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant human ADA protein (ab109839)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
    • Tissue specificity

      Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
    • Involvement in disease

      Defects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
    • Sequence similarities

      Belongs to the adenosine and AMP deaminases family.
    • Cellular localization

      Cell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
    • Target information above from: UniProt accession P00813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 100 Human
      • Entrez Gene: 24165 Rat
      • Omim: 608958 Human
      • SwissProt: P00813 Human
      • SwissProt: Q920P6 Rat
      • Unigene: 654536 Human
      • Unigene: 12689 Rat
      • Alternative names

        • ada antibody
        • ADA_HUMAN antibody
        • ADA1 antibody
        • Adenosine aminohydrolase antibody
        • Adenosine deaminase antibody
        see all

      Images

      • Western blot - Anti-ADA antibody [EPR4438] (ab108352)
        Western blot - Anti-ADA antibody [EPR4438] (ab108352)
        All lanes : Anti-ADA antibody [EPR4438] (ab108352) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Human fetal thymus lysate
        Lane 3 : Rat kidney tissue lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 41 kDa
        Observed band size: 41 kDa

      • Anti-ADA antibody [EPR4438] (ab108352)
        Anti-ADA antibody [EPR4438] (ab108352)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab108352? Please let us know so that we can cite the reference in this datasheet.

      ab108352 has been referenced in 1 publication.

      • Hoppe-Seyler K  et al. Induction of dormancy in hypoxic human papillomavirus-positive cancer cells. Proc Natl Acad Sci U S A 114:E990-E998 (2017). PubMed: 28115701

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