Overview

  • Product name

    Anti-ADAM10 antibody [11G2]
    See all ADAM10 primary antibodies
  • Description

    Mouse monoclonal [11G2] to ADAM10
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Jurkat cell line

  • General notes

    This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab59482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.

Use under nonreducing conditions.

IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificity

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Images

  • IHC image of ab59482 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59482, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Virreira Winter S  et al. Genome-wide CRISPR screen reveals novel host factors required for Staphylococcus aureus a-hemolysin-mediated toxicity. Sci Rep 6:24242 (2016). Read more (PubMed: 27066838) »
  • Riedle S  et al. Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity. Biochem J 420:391-402 (2009). WB . Read more (PubMed: 19260824) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. The antibody ab59482 ADAM10 antibody [11G2] was generated by immunizing mice with the Jurkat T cell line. It recognizes the ADAM10 ectodomain, both mature and immature forms. However, the exact epitope (binding site of antibody to protein) has not been mapped. I hope this information is nevertheless of some help to you. Please do not hesitate to contact us if you need any more advice or information.

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