Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAM10
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for the Propeptide domain of ADAM10 and can detect the latent form of the enzyme. It will not recognize the furin-cleaved form of ADAM10.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide based on the Propeptide domain of human ADAM10. (Peptide available as ab41165.)

  • Positive control

    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab39178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).

Use dilution 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificity

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Images

  • Methanol fixed Hela cells stained with ab39178 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview.

References

This product has been referenced in:

  • Álvarez-Fernández SM  et al. Serological immune response against ADAM10 pro-domain is associated with favourable prognosis in stage III colorectal cancer patients. Oncotarget 7:80059-80076 (2016). ICC/IF ; Human . Read more (PubMed: 27517630) »
  • Xiao Q  et al. Matrix metalloproteinase-8 promotes vascular smooth muscle cell proliferation and neointima formation. Arterioscler Thromb Vasc Biol 34:90-8 (2014). Read more (PubMed: 24158518) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - whole cell (EL4)
Loading amount
100000 cells
Specification
EL4
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 11 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount
20000 cells
Specification
HT1080 Cell line
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 19 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela Cells)
Specification
Hela Cells
Fixative
Methanol
Permeabilization
No

Abcam user community

Verified customer

Submitted Feb 25 2008

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