Anti-ADAM10 antibody - Aminoterminal end (ab39153)

Rabbit polyclonal ADAM10 antibody. Validated in WB, IHC and tested in Mouse, Human. Cited in 3 publication(s). Independently reviewed in 2 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-ADAM10 antibody - Aminoterminal end
    See all ADAM10 primary antibodies
  • Description

    Rabbit polyclonal to ADAM10 - Aminoterminal end
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes the amino end of active ADAM10, and can detect the latent and active forms of the enzyme.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide of Human ADAM10 based on the prohormone convertase (PC) cleavage site. (Peptide available as ab41167.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificity

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Images

  • IHC image of ab39153 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Marcello E  et al. Amyloid-ß Oligomers Regulate ADAM10 Synaptic Localization Through Aberrant Plasticity Phenomena. Mol Neurobiol N/A:N/A (2019). Read more (PubMed: 30989630) »
  • Sogorb-Esteve A  et al. Levels of ADAM10 are reduced in Alzheimer's disease CSF. J Neuroinflammation 15:213 (2018). Read more (PubMed: 30045733) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Kidney)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
Kidney

Dr. Nora Alghamdi

Verified customer

Submitted Nov 20 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Hela Cells)
Loading amount
60000 cells
Specification
Hela Cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 30 2007

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