For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Recently, I bought the antibody anti-ADAM10 (ab39153) to be used in immunohistochemical assays. Before using it, I would like to ask if my calculation is actually correct. The data sheet indicates a working concentration of
5 micrograms/mL for immunohistochemistry. As I can read on the label vial, it indicates that the antibody is at
1 mg/mL. So, can I assume that I should pipet 5 microliters of this vial and take them at a final volume of 1 mL
to get a working concentration of 5 microliters/mL?
Asked on Oct 16 2012
Thank you for your inquiry.
Yes, you are correct. If we supply the antibody at 1 mg/ml and you need to use it at 5 ug/ml that is a 1/200 dilution. 1 mg/ml x ml/.005 mg = 200
If you want to put .005 mL in 1 mL, this would be 1/200.
I hope this information helps. Please contact us with any other questions.
Answered on Oct 16 2012