Recombinant Anti-ADAM10 antibody [EPR5622] (ab124695)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5622] to ADAM10
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ADAM10 antibody [EPR5622]
See all ADAM10 primary antibodies -
Description
Rabbit monoclonal [EPR5622] to ADAM10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ADAM10 (C terminal). The exact sequence is proprietary.
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Positive control
- Jurkat, RAW 264.7, U2OS, LnCaP and MCF-7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.80 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5622 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 84 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. -
Tissue specificity
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Entrez Gene: 29650 Rat
- Omim: 602192 Human
- SwissProt: O14672 Human
- SwissProt: O35598 Mouse
- SwissProt: Q10743 Rat
- Unigene: 578508 Human
see all -
Alternative names
- A disintegrin and metalloprotease domain 10 antibody
- A disintegrin and metalloproteinase domain 10 antibody
- AD 10 antibody
see all
Images
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![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-280814-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: Empty lane
Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 µg)
Lane 4: U20S whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE. Ab124695 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-282779-anti-adam10-antibody-epr5622-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-282717-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.05 µg/ml (Purified)
Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-282715-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.09 µg/ml (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-138473-anti-adam10-antibody-epr5622.jpg)
OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695) -
![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-138474-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa
84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein. -
![Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.com/ps/products/124/ab124695/Images/ab124695-8-benefits-of-recombinant-antibodies.png)
Anti-ADAM10 antibody [EPR5622] (ab124695)
Protocols
Datasheets and documents
References (21)
ab124695 has been referenced in 21 publications.
- Wu JH et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med 12:e10154 (2020). PubMed: 31943789
- Li H et al. Long non-coding RNA CDKN2B-AS1 reduces inflammatory response and promotes cholesterol efflux in atherosclerosis by inhibiting ADAM10 expression. Aging (Albany NY) 11:1695-1715 (2019). PubMed: 30926762
- Brummer T et al. NrCAM is a marker for substrate-selective activation of ADAM10 in Alzheimer's disease. EMBO Mol Med 11:N/A (2019). PubMed: 30833305
- Beerli C et al. Vaccinia virus hijacks EGFR signalling to enhance virus spread through rapid and directed infected cell motility. Nat Microbiol 4:216-225 (2019). PubMed: 30420785
- Liu L et al. A cellular complex of BACE1 and ?-secretase sequentially generates Aß from its full-length precursor. J Cell Biol 218:644-663 (2019). PubMed: 30626721
