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    adam10-antibody-epr5622-ab124695.pdf

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Cell Biology Apoptosis Receptors Receptor Processing
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ADAM10 antibody [EPR5622] (ab124695)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (28)

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Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
  • Anti-ADAM10 antibody [EPR5622] (ab124695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5622] to ADAM10
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
IHC
Silicon Imaging Chamber (1.75 mm) (ab243309)
Protein
Product image
Recombinant Human ADAM10 protein (ab82822)

View more associated products

Overview

  • Product name

    Anti-ADAM10 antibody [EPR5622]
    See all ADAM10 primary antibodies
  • Description

    Rabbit monoclonal [EPR5622] to ADAM10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ADAM10 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, RAW 264.7, U2OS, LnCaP and MCF-7 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.80 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5622
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • ADAM Protein Family
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • ADAM protein family
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ADAMs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Receptor Processing
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ADAM10 protein (ab82822)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP
1/10 - 1/100.

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificity

    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Target information above from: UniProt accession O14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 102 Human
    • Entrez Gene: 11487 Mouse
    • Entrez Gene: 29650 Rat
    • Omim: 602192 Human
    • SwissProt: O14672 Human
    • SwissProt: O35598 Mouse
    • SwissProt: Q10743 Rat
    • Unigene: 578508 Human
    • Unigene: 3037 Mouse
    • Unigene: 42924 Rat
    see all
  • Alternative names

    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Images

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty lane
    Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 µg)
    Lane 4: U20S whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE.  Ab124695 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)

    ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
    Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.05 µg/ml (Purified)

    Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.09 µg/ml (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)
    OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

  • Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
    All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 84 kDa



    84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein.
  • Anti-ADAM10 antibody [EPR5622] (ab124695)
    Anti-ADAM10 antibody [EPR5622] (ab124695)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (28)

Publishing research using ab124695? Please let us know so that we can cite the reference in this datasheet.

ab124695 has been referenced in 28 publications.

  • Liu Y  et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
  • Wu JH  et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med 12:e10154 (2020). PubMed: 31943789
  • Lian B  et al. Hippocampal overexpression of SGK1 ameliorates spatial memory, rescues Aß pathology and actin cytoskeleton polymerization in middle-aged APP/PS1 mice. Behav Brain Res 383:112503 (2020). PubMed: 31981651
  • Mizuno S  et al. ADAM10 is indispensable for longitudinal bone growth in mice. Bone 134:115273 (2020). PubMed: 32062003
  • Lu W  et al. Tetraspanin CD9 interacts with a-secretase to enhance its oncogenic function in pancreatic cancer. Am J Transl Res 12:5525-5537 (2020). PubMed: 33042435
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM10 antibody [EPR5622]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% saponin
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 15 2019

Western blot abreview for Anti-ADAM10 antibody [EPR5622]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
15 µg
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 14 2019

Immunohistochemistry free floating abreview for Anti-ADAM10 antibody [EPR5622]

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (PFA fixed Brain)
Specification
PFA fixed Brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 14 2019

Western blot abreview for Anti-ADAM10 antibody [EPR5622]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary astrocytes)
Loading amount
1 µg
Specification
primary astrocytes
Treatment
LPS 0.1ug/ml, 6h
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 16 2013

Question

I'm interested to buy this antibody but I would like to know with more precision with residues from the Ct domain of the ADAM 10 protein are recognize by this antibody.

Read More

Abcam community

Verified customer

Asked on Mar 08 2013

Answer

The Anti-ADAM10 antibody [EPR5622] (ab124695) has been raised against a synthetic peptide taken from within the region 700 to the C-terminus of the human ADAM10 protein (Uniprot reference O14672).

Read More

Abcam Scientific Support

Answered on Mar 08 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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