Anti-ADAM12 antibody (ab28747)
Key features and details
- Goat polyclonal to ADAM12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM12 antibody
See all ADAM12 primary antibodies -
Description
Goat polyclonal to ADAM12 -
Host species
Goat -
Specificity
This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
AARPLPVSPARALC
, corresponding to N terminal amino acids 2-15 of ADAM12. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. -
Tissue specificity
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Domain
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8038 Human
- Omim: 602714 Human
- SwissProt: O43184 Human
- Unigene: 594537 Human
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Alternative names
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
see all
Images
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Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab28747 has been referenced in 3 publications.
- Chung MW et al. Expression of ADAM12 in Gastric Cancer and its Relation to Tumor Cell Behavior and Prognosis. In Vivo 36:2194-2204 (2022). PubMed: 36099146
- Liu J et al. MicroRNA-144 inhibits the metastasis of gastric cancer by targeting MET expression. J Exp Clin Cancer Res 34:35 (2015). WB . PubMed: 25927670
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P ; Human . PubMed: 19213876