Key features and details
- Goat polyclonal to ADAM12
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAM12 antibody
See all ADAM12 primary antibodies
DescriptionGoat polyclonal to ADAM12
SpecificityThis antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab28747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
1 hour primary incubation is recommended for this product.
FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence.
ab28747 has been referenced in 2 publications.
- Liu J et al. MicroRNA-144 inhibits the metastasis of gastric cancer by targeting MET expression. J Exp Clin Cancer Res 34:35 (2015). WB . PubMed: 25927670
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P ; Human . PubMed: 19213876