Key features and details
- Rabbit polyclonal to ADAM12 - Aminoterminal end
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-ADAM12 antibody - Aminoterminal end
See all ADAM12 primary antibodies
DescriptionRabbit polyclonal to ADAM12 - Aminoterminal end
SpecificityThis antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Synthetic peptide corresponding to Human ADAM12 (N terminal).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody has been peptide affinity purified.
Our Abpromise guarantee covers the use of ab39155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 100 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.|
FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Predicted band size: 100 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
ab39155 has been referenced in 7 publications.
- Huang X et al. Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a. Oncogene N/A:N/A (2018). PubMed: 30042416
- Jiao T et al. Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm. Biomed Res Int 2017:9645874 (2017). WB ; Mouse . PubMed: 28357407
- Mendoza-Parra MA et al. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification. F1000Res 5:54 (2016). PubMed: 27335635
- Brzoska E et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). WB, ICC . PubMed: 25890097
- Archacka K et al. Competence of in vitro cultured mouse embryonic stem cells for myogenic differentiation and fusion with myoblasts. Stem Cells Dev 23:2455-68 (2014). PubMed: 24940624
- Przewozniak M et al. Adhesion proteins--an impact on skeletal myoblast differentiation. PLoS One 8:e61760 (2013). WB, IHC-Fr, ICC/IF ; Mouse, Rat . PubMed: 23671573
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt ; Human . PubMed: 19213876