Key features and details
- Rat monoclonal [6D34] to ADAM15
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2
Product nameAnti-ADAM15 antibody [6D34]
See all ADAM15 primary antibodies
DescriptionRat monoclonal [6D34] to ADAM15
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment corresponding to Mouse ADAM15. Immunized with mouse recombinant protein of A Disintegrin and ADAM15 ectodomain.
RPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLP EALRISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLEN CCYRGRVQGHPSSWVSLCACSGIRGLIVLSPERGYTLELGPGDLQRPVIS RIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVVTETKIVE LVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWT QHNLIEMSSNPAVLLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGM AIQNSICSPDFSGGVNMDHSTSILGVASSIAHELGHSLGLDHDSPGHSCP CPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERQPS LAPMSSLCGNMFVDPGEQCDCGFDECTDPCCDHFTCQLRPGAQCASDGPC CQNCKLHPAGWLCRPPTDDCDLPEFCPGDSSQCPSDIRLGDGEPCASGEA VCMHGRCASYARQCQSLWGPGAQPAAPLCLQTANTRGNAFGSCGRSPGGS YMPCAPRDVMCGQLQCQWGRSQPLLGSVQDRLSEVLEANGTQLNCSWVDL DLGNDVAQPLLALPGTACGPGLVCIGHRCQPVDLLGAQECRRKCHGHGVC DSSGHCRCEEGWAPPDCMTQLKATS
Database link: O88839
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab194610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.|
FunctionActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
Tissue specificityExpressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainThe cytoplasmic domain interacts with endophilin I and sorting nexin 9.
Disintegrin domain binds to integrin alphaV-beta3.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylation increases association with PTKs.
Cellular localizationEndomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
- Information by UniProt
- A disintegrin and metalloproteinase domain 15 (metargidin) antibody
- A disintegrin and metalloproteinase domain 15 antibody
- ADA15_HUMAN antibody
ab194610 has not yet been referenced specifically in any publications.