Recombinant
RabMAb

Recombinant Anti-ADAM15 antibody [EPR5619] (ab124698)

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Overview

  • Product name

    Anti-ADAM15 antibody [EPR5619]
    See all ADAM15 primary antibodies

  • Description

    Rabbit monoclonal [EPR5619] to ADAM15

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ADAM15 aa 250-350 (extracellular). The exact sequence is proprietary.
    Database link: Q13444

  • Positive control

    • SW480 cell lysate; Human colon tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab124698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90, 110 kDa (predicted molecular weight: 93 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use  1/250.00000 - 1/500.00000. See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.

    • Tissue specificity

      Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.

    • Sequence similarities

      Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.

    • Domain

      The cytoplasmic domain interacts with endophilin I and sorting nexin 9.
      Disintegrin domain binds to integrin alphaV-beta3.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    • Post-translational
      modifications

      The precursor is cleaved by a furin endopeptidase.
      Phosphorylation increases association with PTKs.

    • Cellular localization

      Endomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
    • Information by UniProt

    • Database links

      • Alternative names

        • A disintegrin and metalloproteinase domain 15 (metargidin) antibody
        • A disintegrin and metalloproteinase domain 15 antibody
        • ADA15_HUMAN antibody
        • ADAM 15 antibody
        • ADAM metallopeptidase domain 15 antibody
        • Adam15 antibody
        • and cysteine-rich protein 15 antibody
        • Disintegrin and metalloproteinase domain-containing protein 15 antibody
        • disintegrin-like antibody
        • EC 3.4.24. antibody
        • MDC 15 antibody
        • MDC-15 antibody
        • MDC15 antibody
        • Metalloprotease RGD disintegrin protein antibody
        • Metalloproteinase like disintegrin like and cysteine rich protein 15 antibody
        • Metalloproteinase-like antibody
        • Metargidin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified ab124698 at 1:800 dilution (2.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

      • Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution (purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Predicted band size: 93 kDa
        Observed band size: 110,90 kDa
        why is the actual band size different from the predicted?

      • Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution + SW480 cell lysate at 10 µg

        Secondary
        Goat anti-Rabbit HRP

        Predicted band size: 93 kDa



        ab124698 also appears to recognize the pro-protein (110kDa). This image was produced using unpurified antibody.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)

        ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.

      References

      This product has been referenced in:

      • Jana S  et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). Read more (PubMed: 30371322) »
      See 1 Publication for this product

      Publishing research using ab124698? Please let us know so that we can cite the reference in this datasheet.

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