Key features and details
- Rabbit polyclonal to ADAM15 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ADAM15 antibody - N-terminal
See all ADAM15 primary antibodies
DescriptionRabbit polyclonal to ADAM15 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within N terminal amino acids 1-255 of Human ADAM15 (Uniprot ID: Q13444).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)|
|WB||1/500 - 1/3000. Predicted molecular weight: 93 kDa.|
FunctionActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
Tissue specificityExpressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainThe cytoplasmic domain interacts with endophilin I and sorting nexin 9.
Disintegrin domain binds to integrin alphaV-beta3.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylation increases association with PTKs.
Cellular localizationEndomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed.
- Information by UniProt
- A disintegrin and metalloproteinase domain 15 (metargidin) antibody
- A disintegrin and metalloproteinase domain 15 antibody
- ADA15_HUMAN antibody
Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution + NIH 3T3 whole cell lysate
at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle, labelling ADAM15 with ab137387 at 10µg/ml.
ab137387 has been referenced in 1 publication.
- Chatterjee V et al. MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression. PLoS One 9:e110286 (2014). WB, ICC/IF ; Human . PubMed: 25333931