Anti-ADAM17 antibody [1F6] (ab57484)
Key features and details
- Mouse monoclonal [1F6] to ADAM17
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-ADAM17 antibody [1F6]
See all ADAM17 primary antibodies -
Description
Mouse monoclonal [1F6] to ADAM17 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment: RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV , corresponding to amino acids 215-315 of Human ADAM17
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Positive control
- IHC-P: Human small intestine. Flow Cyt: HeLa cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 25/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
1F6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab57484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
(methanol fixed cells) ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. (methanol fixed cells) ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- Unigene: 404914 Human
- Unigene: 27681 Mouse
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Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Images
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ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab57484 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57484, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab57484 has been referenced in 18 publications.
- Garrido MP et al. NGF/TRKA Promotes ADAM17-Dependent Cleavage of P75 in Ovarian Cells: Elucidating a Pro-Tumoral Mechanism. Int J Mol Sci 23:N/A (2022). PubMed: 35216240
- Huang L et al. Rosmarinic acid inhibits proliferation and migration, promotes apoptosis and enhances cisplatin sensitivity of melanoma cells through inhibiting ADAM17/EGFR/AKT/GSK3β axis. Bioengineered 12:3065-3076 (2021). PubMed: 34224305
- Huang H et al. Extracellular domain shedding of the ALK receptor mediates neuroblastoma cell migration. Cell Rep 36:109363 (2021). PubMed: 34260934
- Kaaij MH et al. Transmembrane TNF drives osteoproliferative joint inflammation reminiscent of human spondyloarthritis. J Exp Med 217:N/A (2020). PubMed: 32662821
- He T et al. Inhibition of two-pore channels in antigen-presenting cells promotes the expansion of TNFR2-expressing CD4+Foxp3+ regulatory T cells. Sci Adv 6:N/A (2020). PubMed: 32998896